Table 1.

Different concentrations of sgRNAs and Cas9 protein.

Treatment	Stage	No. of Embryos/Larvae	Min. No. of PGCs	Max. No. of PGCs	Mean	SD	p ***
Control	Neurula	29	12	86	44.14	21.04	0.006679
	22 dpf	14	12	41	26.57	10.30
150/200 **	Neurula	30	0	20	7.23	5.85	0.00616
	22 dpf	20	0	12	5.90	4.78
200/200 **	Neurula	21	0	19	6.90	6.94	0.9672
	22 dpf	19	0	12	3.68	4.06
250/150 **	Neurula	21	0	21	7.23	6.70	0.6031
	22 dpf	18	0	15	5.22	4.85

** sgRNA/Cas9 Concentrations in ng/µl; *** Wilcoxon test