Table 2.

Comparison of CRISPR/Cas9, Dnd1-MO and UV240 methods.

Treatment	Stage	No. of Embryos/Larvae	Minimum No. of PGCs	Maximum No. of PGCs	Mean	SD	p
Control	Neurula	11	15	52	32.00	12.88	0.0004467
	22 dpf	8	51	92	71.88	16.78
CRISPR/Cas9	Neurula	16	0	13	6.00	4.84	0.008104
	22 dpf	11	0	5	1.18	2.04
Dnd1-MO	Neurula	20	0	16	3.30	5.59	0.7032
	22 dpf	9	0	12	5.56	4.77
UV240	Neurula	20	0	16	6.05	5.96	0.3819
	22 dpf	19	0	18	4.89	6.13

Wilcoxon test.