Figure 2.

Messenger RNA (mRNA) isoforms of the EEF1A1 gene. (a) Reference gene model from hg19. Arrows indicate direction of transcription. (b–e) Isoforms and abundances (TPM, transcripts per million reads) discovered in lineage-neg (blue) and total BM (red) cell populations by conventional short-read RNA-seq (b,d) or by full-length RNA-seq (c,e). Open reading frames (ORFs) and a novel protein isoform (arrows) that was confirmed by mass spectrometry for a unique peptide are shown in Figure 3. MatchAnnot confirmed the quality of the transcripts against gencode v19 (details in Methods). Abbreviations: S, short-read RNA-seq; C, canonical transcript and open reading frame (ORF); C*, non-canonical transcript isoforms with canonical ORF. ID#s of the isoforms are from the identifiers generated by the sequencing method. Ensembl IDs are included in Table S8, NCBI Accession numbers in Table S12.