FA-induced membrane currents recorded from glioma cells. (A) Summary of currents recording from GL261, A172 and U87 glioma cells using whole-cell voltage clamp (held at −60 mV), elicited by addition of FA (100 µM) to the extracellular solution (pH 6.0). (B) Representative current recording and the summary of currents (C) from GL261 glioma cells using whole-cell voltage clamp (held at −60 mV), elicited by addition of FA (100 µM) to the extracellular solution (pH 6.0). The recording was performed with or without extracellular addition of FA-Cyt c NPs, given at different concentrations. (D) Summary of currents recorded from mock-transfected GL261 cells and cells transfected with siRNA against the PCFT, both induced by application of FA. Error bars represent SEM. * indicates a significant difference (p < 0.05). n is the number of cells investigated.