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Bee venom PLA2 binding pocket, demonstrating the binding of the PL-methotrexate complex. For clarity, only residues within 3.0 A of the Ca2+ metal center are shown. The Ca2+ ion is ligated to tryptophan, 2 glycine, and aspartate residues, as well as the phosphate group of the PL-methotrexate conjugate. Atoms are labeled as follows carbon (cyan), hydrogen (white), oxygen (red), nitrogen (blue), phosphate (gold), and calcium (yellow). The protein backbone is shown as a cyan ribbon.
