Skip to main content
. 2019 Mar 25;6(10):1801862. doi: 10.1002/advs.201801862

Figure 5.

Figure 5

PLCL1 repressed ccRCC progression and promoted tumor cell “slimming” through UCP1‐mediated lipid browning. On the basis of PLCL1 overexpression, cells with UCP1 knocked down were constructed by transfecting UCP1 siRNA. We constructed four groups of cell lines for rescue experiments, including cell lines with PLCL1 expression lentivirus control vector and siUCP1 control, cell lines with PLCL1 expression lentivirus and siUCP1 control, cell lines with PLCL1 expression lentivirus control vector and siUCP1, and cell lines with PLCL1 expression lentivirus and siUCP1. A) Western blot assay for the protein levels of PLCL1 and UCP1 in indicated cells. B) Cell growth curves of CCK8 assays for indicated cells. ***, p < 0.001, **, p < 0.01, p = ns (no significance). C) Migration and invasion assay for indicated ccRCC cells (Magnification: 200×). t‐test, ***, p < 0.001, **, p < 0.01, *, p < 0.05, p = ns (no significance). D) Photomicrographs of Oil Red O staining of indicated cells (Magnification: 400×). Relative diameters of lipid droplets and the ccRCC cells described above.