Figure 6.

PLCL1 regulated the protein stability of UCP1 by affecting UCP1 ubiquitination levels in ccRCC. A) Relative mRNA levels of UCP1 in the cells PLCL1‐overexpressing and PLCL1‐knockdown cells. p = ns (no significance). B,C) ccRCC cells with stable PLCL1 knockdown and overexpression were treated with cycloheximide (CHX) at the indicated time points. Cells were collected, and UCP1 protein expression was analyzed by western blot. D) Cells with stable PLCL1 knockdown were treated with vehicle (DMSO), MG132 (20 × 10⁻⁶ m) or chloroquine (50 × 10⁻⁶ m) for 12 h. Western blotting was used to analyze the protein level of UCP1. E) GO enrichment for the indicated cells based on the results from sequencing. F,G) The cells with stable PLCL1 knockdown and overexpression were lysed and subjected to immunoprecipitation with an antibody against UCP1 and analyzed by western blotting with an anti‐ubiquitin antibody.