FIGURE 4.

Functional characterization of PsRPs26 during the interaction between wheat and stripe rust using host-induced gene silencing. (A) Phenotypic changes in the fourth leaves of plants pre-inoculated with the positive control vector (BSMV-PDS), FES buffer (CK), or empty BSMV vector (BSMV:γ). Phenotypes for the fourth leaves inoculated with the P. striiformis f. sp. tritici (Pst) CYR31 race at 14 dpi. (B) Relative transcript levels of PsRPs26 in the silenced leaves. BSMV:γ leaves infected with the Pst CYR31 race were used as a control. (C) Quantification of uredinial density in PsRPs26 knockdown plants 14 dpi with the CYR31 isolate. Means and standard deviations were calculated from three independent replicates. Significant differences were determined using Student’s t-test: ^∗P < 0.05.