Figure 1.

Inhibition of SUMO pathway partially restored DAPK1 protein levels at the presence of TSC2. (A) HEK293T was transfected with HA-DAPK1, Flag-TSC2, and different E2 siRNAs. (B,C) HEK293T cells were transfected with HA-DAPK1, LacZ, TSC2 and four different SENPs as indicated. Cell lysates were extracted 48 h post-transfection and immunoblotted with respective antibodies. The intensity of the bands was quantified using LICOR Odyssey software and represented by bar graphs. The experiments were repeated three times (n = 3), and representative images are presented. The representative western blot images are from different gels and each lane was loaded with the same amount of proteins. Data of triplicate assays are presented as mean ± S.D. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance. “+” indicated that the plasmid was transfected, “−” indicated that the plasmid was not transfected.