Figure 5.

SUMO pathway did not regulate DAPK1 protein levels via direct conjugation. (A) A diagram illustrating the panel of DAPK1 (1–364) point mutants. (B) HEK293T cells were transfected with LacZ, SENP6 and the DAPK1 point mutants as indicated. (C–E) HEK293T transfected with LacZ, DAPK1 (1–364) point mutants were exposed to 20 μg/mL CHX for 0–8 h as indicated and the statistical results were summarized in (F). The intensity of the bands was quantified using LICOR Odyssey software and represented by bar graphs. The experiments were repeated three times (n = 3) and representative images are presented. The representative western blot images are from different gels and each lane was loaded with the same amount of proteins. Data of triplicate assays are presented as mean ± S.D. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance. “+” indicated that the plasmid was transfected, “−” indicated that the plasmid was not transfected.