Skip to main content
. 2019 Apr 8;8(4):327. doi: 10.3390/cells8040327

Figure 2.

Figure 2

Characterization of CRISPR/Cas9-edited GLA-null hESC clones. (A) Immunofluorescence staining demonstrating the protein expression of pluripotency markers OCT4, TRA-1-60, NANOG and TRA-1-81 in GLA-null hESC clones #19 and #27. Nuclei stained with DAPI. (B) Embryoid body formation assay showing differentiation of GLA-null hESC clones #19 and #27 into three germ layers: endoderm immunoreactive for HNF3β, mesoderm immunoreactive for α-SMA, and ectoderm immunoreactive for nestin. Nuclei stained with DAPI. (C) Representative karyograms GLA-null hESC clones #19 and #27. Visualization was performed using Agilent CytoGenomics software.