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. 2019 Apr 8;8(4):327. doi: 10.3390/cells8040327

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Proteomic analysis of GLA-null CMs. (A) The most enriched cellular component GO terms in the list of 60 genes downregulated in GLA-null CMs. (B) Visualization of the most enriched cellular component GO terms using REVIGO software. (C) Western blot showing expression of the mediators of vesicular trafficking in GLA-null CMs #19 and #27 as compared to wild type control (H9). GAPDH served as a loading control. (D) Quantitation of expression of proteins annotated as the mediators of vesicular trafficking. The data are presented as mean ± standard deviation error bars from three independent experiments. (D) Flow cytometric analysis of CD63 expression in H9- and GLA-null CM-derived exosome isolated with magnetic beads directly from cell culture medium. (E) Quantification of exosome numbers in the supernatant from H9 and GLA-null CMs.