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. 2018 Dec 6;17(6):1069–1080. doi: 10.1111/pbi.13038

Figure 1.

Figure 1

The TMV vector system, which allows Gibson assembly of the ORF of interest. Schematic representation of intermediate entry plasmids pMTMVi‐N and pMTMVi‐M, and the Gibson assembly reactions that allow insertion of the gene of interest (GoI) into pGTMV to produce the binary plasmids that will express the recombinant viruses (pGTMVn‐GoI and pGTMVm‐GoI). Ori pUC and Ori pSa, replication origins; AmpR and KanR, ampicillin and kanamycin resistance selection markers; LacZ, fragment of E. coli b‐galactosidase, which allows blue–white screening in the presence of X‐gal; TMV and 3′ ToMV, viral cDNAs; LB and RB, left and right borders of the Agrobacterium. tumefaciens transfer DNA; P35S and T35S, Cauliflower mosaic virus (CaMV) 35S promoter and terminator; δ, ribozyme.