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. 2018 Dec 6;17(6):1069–1080. doi: 10.1111/pbi.13038

Figure 4.

Figure 4

Equivalence of antifungal activity for the in planta‐and in fungus‐produced AfpB. (a) Purification of AfpB from Nicotiana benthamiana leaves by cationic exchange chromatography. Coomassie‐blue stained tricine‐SDS‐PAGE gel loaded with equivalent volumes of extracelular fluid (ECF), flow‐through (FT) and different eluted chromatographic fractions (1, 5, 13‐16), and 3.5 μg of Penicillium digitatum purified protein (AfpB[Pd]). (b‐c) In vitro inhibitory activity of AfpB against P. digitatum fungus. Dose–response curves comparing the fungal growth inhibitory activity of the purified AfpB[Pd] and N. benthamiana AfpB (AfpB[Nb]) (b), or with ECFs from leaves of N. benthamiana producing AfpB (AfpB[Nb] ECF) or GFP (GFP[Nb] ECF) (c). Data shown represent the mean ± SD of three biological replicates after 72 h of incubation with the indicated amounts of AfpB.