Figure 6.

Characterization of the (SP4) ₁₈ ‐EGFP and (SP) ₃₂ ‐EGFP transgene products in the hairy roots treated with BFA. (a–h) Fluorescence micrographs of the cultured hairy roots. Root cells were captured in the cortical cytoplasm under green fluorescence channel (488 nm excitation with 525/50 nm filter) with a 40× water‐immersion objective. A substantial fluorescence signal in a reticulate network throughout the cortical cytoplasm was indicative of ER networks labelled with fluorescent transgene products. Scale bar = 50 μm; (i, j) Western blotting detection of the (SP4) ₁₈ ‐EGFP and (SP) ₃₂ ‐EGFP transgene products, respectively, accumulated in hairy roots. Fifteen μL of clarified root extracts was loaded into each well. Crtl⁺: EGFP standard (50 ng); A new glycoform of (SP)₃₂‐EGFP (~60 kDa) formed in the BFA‐treated hairy roots is indicated by the arrow.