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. 2018 Dec 14;17(6):1130–1141. doi: 10.1111/pbi.13043

Table 1.

Recombinant EGFP yields of the hairy root cultures in liquid media for 18 days

Protein expressed Media (mg/L) Roots (mg/gFW) Total accumulated protein (mg/L) Secreted (%)
(SP4)18‐EGFP 28.2 ± 3.4a 0.028 ± 0.001a 32.0 88.1
(SP)32‐EGFP 24.6 ± 2.5a 0.009 ± 0.005b 25.8 95.3
EGFP 0.5 ± 0.2b 0.025 ± 0.004a 2.8 17.9

Each value represents the mean of five selected hairy root lines ± SD (n = 5). Different letters (a, b) indicate significant difference as determined by a one‐way ANOVA followed by Tukey post hoc range test (< 0.05).

The EGFP equivalent of the (SP)32‐EGFP and (SP4)18‐EGFP fusion proteins was quantified.

The proteins accumulated in both root tissues and culture media.