Table 1.
Protein expressed† | Media (mg/L) | Roots (mg/gFW) | Total accumulated protein (mg/L)‡ | Secreted (%) |
---|---|---|---|---|
(SP4)18‐EGFP | 28.2 ± 3.4a | 0.028 ± 0.001a | 32.0 | 88.1 |
(SP)32‐EGFP | 24.6 ± 2.5a | 0.009 ± 0.005b | 25.8 | 95.3 |
EGFP | 0.5 ± 0.2b | 0.025 ± 0.004a | 2.8 | 17.9 |
Each value represents the mean of five selected hairy root lines ± SD (n = 5). Different letters (a, b) indicate significant difference as determined by a one‐way ANOVA followed by Tukey post hoc range test (P < 0.05).
The EGFP equivalent of the (SP)32‐EGFP and (SP4)18‐EGFP fusion proteins was quantified.
The proteins accumulated in both root tissues and culture media.