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. 2019 Apr 1;9(4):497. doi: 10.3390/nano9040497

Figure 4.

Figure 4

Osteogenesis on the Alginate/FmocFF composite hydrogel. (a) Quantification of Alkaline Phosphatase (ALP) activity of MC3T3-E1 preosteoblast cells 3 days after seeding on Alginate/FmocFF composite hydrogel and following 14 days of osteogenic differentiation. (b) Quantification of calcification by Alizarin red staining of MC3T3-E1 preosteoblast cells 3 days after seeding on Alginate/FmocFF composite hydrogel and following 14 days of osteogenic differentiation. (c,d) Optic microscope images of MC3T3-E1 preosteoblast cells stained with Alizarin red (c) 3 days after seeding on Alginate/FmocFF composite hydrogel and (d) following 14 days of osteogenic differentiation. Scale bar = 500 µm. (e) Relative mRNA expression of osteogenic genes (ALP, OCN, RUNX2, BMP-2, and Col-I) was quantitated by RT-qPCR analysis of MC3T3-E1 cells 3 days after seeding on Alginate/FmocFF composite hydrogel and following 14 days of osteogenic differentiation.