Table 4.
Comparison of skin permeation of HQ formulations (F) across synthetic (S.M. for Strat-M®) or natural (M.S. for Mouse skin) membranes. Key: Jss, steady-state transdermal flux calculated from the slope of the Cartesian plot of the cumulative amount of the drug present in the receptor compartment versus time; ER, enhancement ratio, calculated as the ratio of steady-state transdermal flux from each formulation compared to formulation 1; P, permeability coefficient calculated by using formula Jss/cd (cd is the amount of drug applied in the donor compartment, so 200 mg of formulation is equivalent to 10 mg of HQ); D, diffusion coefficient (cm2/h) calculated by using formula Jss = d.k/h × cd (where h is the thickness of the Strat-M® or mouse skin); NA, not applicable.
| F | Membrane | Jss (µg/cm2/h) | Lag time (h) | P (cm/h) × 102 | D (cm2/h) × 103 | ER | Amount of HQ in the Skin (mg/g) |
|---|---|---|---|---|---|---|---|
| F1 | S.M. | 49.3 ± 2.4 | 0.95 ± 0.06 | 0.49 ± 0.02 | 0.54 ± 0.03 | - | NA |
| F2 | S.M. | 39.3 ± 3.9 | 1.18 ± 0.09 | 0.39 ± 0.03 | 0.43 ± 0.04 | 0.8 ± 0.1 | NA |
| F3 | S.M. | 106.3 ± 6.5 | 0.47 ± 0.08 | 1.06 ± 0.08 | 1.17 ± 0.09 | 2.2 ± 0.2 | NA |
| F4 | S.M. | 78.1 ± 3.3 | 0.79 ± 0.07 | 0.78 ± 0.03 | 0.85 ± 0.03 | 1.6 ± 0.1 | NA |
| F1 | M.S. | 309.9 ± 66.3 | 0.35 ± 0.08 | 3.09 ± 0.66 | 4.31 ± 0.92 | - | 3.58 ± 1.21 |
| F2 | M.S. | 221.5 ± 43.1 | 0.42 ± 0.08 | 2.21 ± 0.42 | 3.10 ± 0.06 | 0.7 ± 0.1 | 2.50 ± 1.43 |
| F3 | M.S. | 1754.1 ± 184.9 | 0.12 ± 0.06 | 17.5 ± 1.82 | 24.5 ± 2.58 | 5.7 ± 0.6 | 1.36 ± 0.84 |
| F4 | M.S. | 700.5 ± 213.0 | 0.12 ± 0.03 | 7.00 ± 0.21 | 9.81 ± 0.25 | 2.3 ± 0.7 | 1.57 ± 0.78 |