Figure 5.

Plasma β-hydroxybutyrate levels (mM) of fed from hatch and delayed fed broiler chicks. Mean (n = 6 males/treatment) plasma β-hydroxybutyrate levels. β-hydroxybutyrate was measured using a β-hydroxybutyrate (ketone body) colorimetric assay kit (Cayman Chemical). Error bars denote standard deviations. Significant (p < 0.05) differences between treatment groups were determined using analysis of variance. *** p < 0.0001 denotes a significant difference between fed at hatch chicks and chicks delayed access to feed for 48 h.