Figure 6.

Delayed feeding of peri-hatch broiler chicks alters hepatic miRNA expression. (a–d) Real-time quantitative PCR (RT-qPCR) was utilized to measure the expression of hepatic miRNA expression. Values are shown as the mean ΔCt (n = 6 males) for each treatment group (fed at hatch or delayed) at each time point. MiRNA expression levels were normalized to snoU83B levels. Error bars denote standard deviations. Significant (p < 0.05) differences in expression were determined using analysis of variance. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences between fed at hatch chicks and chicks with delayed access to feed for 48 h.