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. 2019 Apr 25;8(4):380. doi: 10.3390/cells8040380

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ectopic activation of Cre drivers in hepatocytes after liver injury. (a–c) Schematic experimental outline. Mice were injected with CCl₄ after a three-day washout (a), a three-week washout (b) or no washout (c) after tamoxifen and sacrificed three days later. The percentage of tdTomato⁺ Hnf-4α⁺ cells was quantified in liver sections of Ck19-CreER-tdTomato and Opn-CreER-tdTomato mice (n = 3–5; 18 pictures per mouse). (d) Immunofluorescent detection of Hnf-4α and tdTomato on liver sections of mice treated with tamoxifen and CCl₄ without washout period. Scale bars: 50 μm.