Figure 7.

Effect of NGF or NGF(1-14) peptides on Erk1/2 and Akt phosphorylation. Representative western blotting (A,C) and densitometric analyses (B,D) of pErk1/2 and pAkt in PC12 cells treated for 15 min with 50 μM NGF(1-14) peptides or 50 ng/mL NGF protein in the absence (☐) or in the presence of 1 μM CuSO₄ (■) or in 50 μM BCS 24 h pre-treated medium (■). Data obtained from at least three independent experiments. The phosphorylated level of Erk1/2 and Akt was reported as ratio over the corresponding total protein (*** p < 0.001 versus control; §§§ p < 0.001 versus CuSO₄; ### p < 0.001 versus BCS; one-way Anova).