Figure 8.
Effect of NGF or NGF peptides on AMP response element-binding protein (CREB) phosphorylation. Representative western blotting (A,B) and densitometric analyses (C) of pCREB in PC12 cells treated for 15 min with 50 μM NGF peptides or 50 ng/mL NGF protein in the absence (☐) or in the presence of 1 μM CuSO4 (■) or in 50 μM BCS 24 h pre-treated medium (■). Data obtained from at least three independent experiments. The phosphorylated level of CREB was reported as ratio over the corresponding total protein (** p < 0.01, *** p < 0.001 versus control; §§§ p < 0.001 versus CuSO4; ### p < 0.001 versus BCS; one-way Anova).