Figure 1.

Breast cancer cells with decreased ZNF143 show better survival in glucose- and/or FBS-deprived conditions, which are chloroquine-dependent. (A,B) MCF7 sh-Control and sh-ZNF143 cells were grown in four different conditions for 24 h, and viable cells were counted by fluorescence-activated cell sorting. G and F denote glucose and FBS, respectively. (C–E) Cells were plated on 96-well plates and grown for 24 h. The cells were then maintained in four different conditions in terms of FBS and glucose, and cell survival or growth was monitored by capturing images every 2 h over 4 days. Cells were maintained in the presence of 10 μM chloroquine (D), 100 nM Wortmannin (E), or dimethysulfoxide (vehicle). Relative confluency is shown in the graphs. Data are expressed as means ± S.E. of at least three independent experiments. Statistical significance was assessed using paired Student’s t-tests (* p < 0.05 and ** p < 0.005). Results shown are representative of at least three independent experiments.