Figure 2.

More autophagic vesicles are observed in ZNF143 knockdown cells than in control cells. (A) Growing cells were maintained in growing media or fetal bovine serum-free media for 24 h and then fixed for TEM. (B,C) cells were grown on coverslips for 24 h and then exposed to four different media for an additional 24 h to monitor autophagic processes by autophagosome-selective marker labeling in live cells. Foci were visualized by confocal microscopy, quantified by using ImageJ software, and statistically analyzed by GraphPad software (C). Results shown are representative of at least three independent experiments.