Figure 5.

NQO1 is reduced in ZNF143-silenced breast cancer cells, and is important for p53 stability. (A) Growing cells were harvested and subjected to liquid chromatography–mass spectrometry to profile altered proteins by ZNF143 knockdown. Approximately 5000 proteins were profiled, and 177 proteins were significantly altered for pathway analysis. (B) Growing cells were harvested for immunoblotting to determine the expression of NQO1. (C) Growing cells were transfected with a vector (pFLAG-CMV) or a plasmid encoding the full length ZNF143 (hZNF143FL) for 24 h and harvested for immunoblotting. (D) Growing cells were harvested for ROS measurements by flow cytometry. Cells were incubated with 10 μM H₂DCFDA for 10 min before harvesting. (E) Growing cells were harvested for RT-PCR to determine the expression of NQO1, PTGR1, and KYNU at the mRNA level. Results shown are representative of at least three independent experiments.