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. 2019 Apr 10;8(4):338. doi: 10.3390/cells8040338

Figure 3.

Figure 3

miR-130a regulates ATG5 expression and HCV replication. miR-130a mimic or miR-130a gRNA and their corresponding negative control were transfected to Huh7.5.1 cells and Huh7.5.1 cells infected with JFH1 HCV infection at a multiplicity of infection (MOI) of 0.2 (JFH1 cells). Total RNA or protein of the cells was harvested at 72 h post vector transfection and 48 h post JFH1 HCV infection. (A) Level of miR-130a overexpression; (B) miR-130a overexpression reduces ATG5 mRNA levels in Huh7.5.1 and JFH1 cells; (C) miR-130a overexpression inhibits HCV RNA expression in JFH1 cells; (D) miR-130a mimic reduces ATG5 and HCV core protein levels in Huh7.5.1 and JFH1 cells; (E) miR-130a gRNA knocks down miR-130a expression in Huh7.5.1 and JFH1 cells; (F) miR-130a gRNA increases ATG5 mRNA levels; (G) miR-130a gRNA promotes HCV RNA expression; and (H) miR-130a mimic and miR-130a gRNA transfection does not affect cell viability. Data are expressed as mean ± SD of three sample replicates. ** p < 0.01, and *** p < 0.001.