Table 1.
Potential off-target sites predicted by CCTop—CRISPR/Cas9 target online predictor software.
| Name | Coordinates | MM | Target Sequence | PAM |
|---|---|---|---|---|
| Dut | chr2:125247853-125247874 | 0 | CGCGCGCGGAC[CCGCGGGT] | GGG |
| Off-1 | chr13:88680616-88680637 | 4 | TGAGTGGGGAC[CCGCGGGT] | TGG |
| Off-2 | chr4:120746882-120746903 | 4 | CTGGAGCGGCC[CCGCGGGT] | GGG |
| Off-3 | chr15:28025084-28025105 | 4 | CGGCCACGGCC[CCGCGGGT] | AGG |
| Off-4 | chr11:23306808-23306829 | 4 | GGGGCGGGGAG[CCGCGGGT] | GGG |
| Off-5 | chr2:118372992-118373013 | 4 | CGCTGGTGGCC[CCGCGGGT] | TGG |
| Off-6 | chr5:64803646-64803667 | 4 | ACCGCACGGAC[GCGCGGGT] | GGG |
| Off-7 | chr18:85179644-85179665 | 3 | CGTGCGCGCAC[GCGCGGGT] | GGG |
| Off-8 | chr9:77319229-77319250 | 4 | CGCGCTTACAC[CCGCGGGT] | GGG |
| Off-9 | chr2:104319532-104319553 | 3 | CGCGTGCGCAC[ACGCGGGT] | AGG |
| Off-10 | chr19:36918725-36918746 | 4 | CTCGCTGGGAC[GCGCGGGT] | AGG |
| Off-11 | chr5:75044665-75044686 | 4 | TGGGCGCGGGC[GCGCGGGT] | GGG |
| Off-12 | chr4:152086570-152086591 | 4 | CGCACCCAGAC[ACGCGGGT] | CGG |
| Off-13 | chr3:41563582-41563603 | 4 | GGCGCGGGGGC[GCGCGGGT] | CGG |
| Off-14 | chr8:60640130-60640151 | 4 | GGCGCGTGGGC[ACGCGGGT] | TGG |
| Off-15 | chr9:40192333-40192354 | 3 | CGCGCGGGGCC[CAGCGGGT] | CGG |
| Off-16 | chr2:174438958-174438979 | 4 | AGCGCGTGGGC[CTGCGGGT] | CGG |
| Off-17 | chr17:88792070-88792091 | 4 | CGGGCGGGGGC[CGGCGGGT] | GGG |
| Off-18 | chr2:28641663-28641684 | 4 | G GCACGGGGAC[CCGGGGGT] | GGG |
| Off-19 | chr17:28350853-28350874 | 4 | GGCGGGCGGGC[CCACGGGT] | GGG |
| Off-20 | chr5:107597539-107597560 | 4 | GGCGCGTGGAT[CGGCGGGT] | AGG |
The table presents the chromosomal location, the number of mismatches (MM), the target, and the adjacent PAM sequences. The first row depicts the target site of the designed gRNA, further rows list the top 20 candidates for off-target sites. Mismatches are indicated in red. Brackets include core sequences [34]. The genomic segments that were successfully sequenced are shown in bold.