Figure 2.

Differential protein interactions detected for peptides mimicking wt- and mutated AFT motifs. (A) Pull-down of proteins from cystic fibrosis bronchial epithelial (CFBE) parental cells that bind to non-conjugated beads (control) and beads conjugated to peptides corresponding to mutated and non-mutated AFTs. In total, 3393 proteins were identified by the LC-MS/MS method. AFT interactors were quantified using intensity-based absolute quantification (iBAQ) values and compared using the ratio log₂(mutated AFT interactors/non-mutated AFT interactors); −5 ˂ log₂ ˂ 5 were considered non-specific proteins. Experiments were performed in triplicate (N = 3). (B) Biological process and (C) cellular component of proteins with increased affinity to mutated vs. non-mutated AFTs. DAVID was used to evaluate the enriched GO terms in the 114 proteins with increased affinity to mutated vs. 742 proteins with increased affinity to non-mutated AFTs. −log₁₀(p-value) was considered to evaluate the statistical significance for all categories.