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Experimental design. Top (NaCl): Ischemia-reperfusion (IR) animals underwent 2 h of unilateral hind limb tourniquet ischemia (red bar), followed by 2 h of reperfusion (open bar). The right, non-ischemic hind limb served as a control, and Nacl was administered ip 30 min before ischemia. Bottom (Sildenafil + IR): the same protocol was performed, but 1mg/kg sildenafil/NaCl ip was administered.
