Figure 5.

Effect of FA-PEG-modification of cationic liposomes on association of siRNA with FA-PEG-modified cationic liposomes. siRNA association by cationic liposomes was analyzed by gel retardation assay. (A) LP-HAPC-1–3mol%PEG₂₀₀₀, LP-HAPC-1mol%PEG₅₀₀₀, LP-HAPC-1–3mol%FA-PEG₂₀₀₀, and LP-HAPC-1mol%FA-PEG₅₀₀₀, (B) LP-OH-1–3mol%PEG₂₀₀₀ and LP-OH-1–3mol%FA-PEG₂₀₀₀, (C) LP-OH-C-1–3mol%PEG₂₀₀₀ and LP-OH-C-1–3mol%FA-PEG₂₀₀₀ were used. Each liposome was formed with siRNA at various charge ratios (+:−) from 1:1 to 4:1, and were analyzed using 18% acrylamide gel electrophoresis.