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. 2019 Apr 15;11(4):181. doi: 10.3390/pharmaceutics11040181

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of FA-PEG-modification of cationic liposomes on association of siRNA with FA-PEG-modified cationic liposome. siRNA association by cationic liposomes was analyzed by exclusion assay using an SYBR^® Green I Nucleic Acid Gel Stain. (A) LP-HAPC-1–3mol%PEG₂₀₀₀, LP-HAPC-1mol%PEG₅₀₀₀, LP-HAPC-1–3mol%FA-PEG₂₀₀₀, and LP-HAPC-1mol%FA-PEG₅₀₀₀, (B) LP-OH-1–3mol%PEG₂₀₀₀ and LP-OH-1–3mol%FA-PEG₂₀₀₀, (C) LP-OH-C-1–3mol%PEG₂₀₀₀ and LP-OH-C-1–3mol%FA-PEG₂₀₀₀ were used. The siRNA lipoplexes were formed at various charge ratios (+:−) from 1:1 to 4:1. As a control, the value of fluorescence obtained upon addition of free siRNA solution was set as 100%. The amount of siRNA available to interact with the SYBR^® Green I was expressed as a percentage of the control. Each column represents the mean + SD (n = 3).