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Perforin production by CD8+ T-cells remained unaffected by the co-culture with any macrophage subset. (a) The concentration of perforin released into culture supernatants of isolated autologous CD8+ T-cells cultured with MDM subsets for 48 h was quantified by an immunobead assay (n = 3). (b) The proportion of perforin+ CD8+ T-cells in co-cultures with macrophage subsets is shown (n = 7).
