Figure 2.

Genomic structures of seven crested ibis MHC haplotypes. A total of seven haplotypes were inferred from genotype data, and their genomic structures of class II region were further determined by La-PCR using representative homozygotes. Dark and light grey boxes indicate the IIα and IIβ genes, respectively, with numbers indicating different alleles on the full-length level. Black boxes indicate the major class I gene (UAA) and white boxes indicate other functional genes [20]. Haplotypes are aligned, with bracketed dash lines representing deleted regions (the lengths were denoted aside) in relative haplotypes. Dotted lines between BRD2 and UAA represent the unsequenced region. Locations of the La-PCR fragments amplified by the six reported sets of primers (P1–P4, P6, and P7) [20] are marked below the haplotypes.