Figure 1.
(a) Ponceau S staining of human transthyretin (huTTR) treated or non-treated (vehicle) with bioactives after electroblotting of SDS-PAGE onto the nitrocellulose membrane (above). Protein: Drug ratio of 1:10. Nitroblue tetrazolium (NBT)/glycinate staining of the membrane above after de-staining Ponceau S (below). (b) Displacement of bound 8-anilino-1-naphthalene sulfonic acid from huTTR by increasing concentrations of Centella asiatica (CAB). The bar chart columns and error bars represent the means of triplicates and standard deviations, respectively.