Skip to main content
. 2019 Mar 29;9(4):128. doi: 10.3390/biom9040128

Figure 2.

Figure 2

(a) Resistance to urea-mediated denaturation of huTTR in the presence and absence of Centella asiatica bioactive (CAB) components. HuTTR (0.07 µg/µL) was incubated in the presence of CAB (6.67 µg/µL) dissolve in GMSO or in the presence of GMSO only. (b) Bar chart represents the means of huTTR with and without CAB (protein: CAB mass ratio 1:100) and with their standard errors. * values are significantly different at p < 0.05. (c) Tricine SDS-PAGE gel (10%) of huTTR with increasing concentrations of CAB subjected to urea-denaturation stress and cross-linked with glutaraldehyde. M: Protein molecular weight marker; 1: 0 M urea + GMSO only; 2: 7 M urea + GMSO only; 3: 7 M urea + CAB (3.33 µg/µL); 4: 7 M urea + CAB (6.66 µg/µL); 5: 7 M urea + CAB (13.33 µg/µL).