Figure 1.

α2,8-sialylated particles reduce the production of ROS and the depolarization of the membrane when neutrophils are stimulated with PMA. (A) Scatter plot of the relative amount of ROS (%) produced after 30 min of stimulation with PMA. The determination of the relative amount of ROS (%) was performed using DHR. The values for PMA-treated cells were set to 100%. Four independent experiments were performed. (B) Scatter plot of the polarization status of the membrane. Determination of membrane depolarization was performed using DiBAC₄. The emitted fluorescence of the PMA-induced cells was set to 100%. Mean values (n ≥ 3) and standard deviations are displayed in the diagrams. ANOVA and a multiple-comparison Tukey test were applied. Statistically significant differences are given as follows: ns, not significant, ** p ≤ 0.01; *** p ≤ 0.001; and **** p ≤ 0.0001.