FIGURE 1.

LecRK-I.8 is involved in Arabidopsis response to insect eggs. (A) LecRK-I.8 expression 72 h after application of P. brassicae egg extract (EE) in Col-0 and lecrk-I.8 T-DNA mutant. Untreated plants were used as control (C). Significant difference between control and treatment is indicated (Student’s t-test, ^∗∗P < 0.01). n.d., not detected. Mean ± SE of three technical replicates are shown. This experiment was repeated twice with similar results. (B) Natural deposition of P. brassicae eggs (left) or application of 2 μl of P. brassicae EE (right) onto a leaf of pLecRK-I.8::NLS-GFP-GUS line. GUS expression was analyzed by histochemical staining 96 h after treatment. Arrowheads indicate the site of oviposition and EE application. Bar = 1 mm (C) PR1 expression 72 h after P. brassicae EE treatment. #1 and #2 are two independent lines where lecrk-I.8 was complemented with a LecRK-I.8-Venus construct. Different letters indicate significant differences (ANOVA followed by Tukey’s honest significant difference test, P < 0.05). Mean ± SE of three technical replicates are shown. This experiment were repeated once with similar results. (D) PR1 expression 72 h after treatment with EE from Pieris brassicae (P.b.) or Spodoptera littoralis (S.l.) in Col-0 (black bars) and leckrk-I.8 (white bars). Untreated plants were used as control (C). Significant differences between control and treatment are indicated (Student’s t-test, ^∗∗∗P < 0.001). Mean ± SE of three technical replicates are shown. This experiments were repeated twice with similar results.