Figure 3.

Therapeutic efficacy of vaccine and combination (vaccine plus Doxil) treatment was evaluated and compared with the control and Doxil (as the cytotoxic positive control) in a mouse model of 4T1 breast tumor. Values are presented as the mean ± SD (n = 5 per group). A – Animal body weight was measured every 2 days. *A significant difference (p < 0.05) of body weight loss between control group with Doxil and combination from day 17, as well as between all groups from day 30 up to day 60. B – Tumor volume (mm3) was measured every 2 days. *A significant difference (p < 0.05) of average tumor volume between control group with Doxil and combination at days 37, 39, 42, 44. C – Kaplan-Meier curves show the survival rate of treatment and control group. *A significant difference (p < 0.05) between control group with Doxil and combination group. D – Measuring the time to reach endpoint (TTE) or the time to reach tumor volume above 1000 mm3 showed a significant difference between control group with combination and Doxil group