Fig. 2.

MiR-107 represses the proliferation, migration and invasion of SW620 cells. a MiR-107 suppresses the proliferation of SW620 cells. The cells were treated with NC mimic or miR-107 mimic for the times shown. Cell viabilities were detected using the MTT assay. b and c MiR-107 inhibits the horizontal migration of SW620 cells. The effect of miR-107 cells on horizontal migration was evaluated with a wound-healing assay. The confluent cells were starved with serum-free medium for 6 h and then scratched with 10-μl pipette tips. After washing with PBS, the cells were treated with or without miR-107 mimic for another 8 h. The images were taken at 0 h and 8 h in the same field with an Olympus IX70 inverted microscope. Representative images (100× magnification) and quantitative data are shown in (b and c), respectively. d and e MiR-107 supresses the vertical migration and invasion abilities of SW620 cells. The effect of miR-107 on vertical migration and invasion was assessed with transwell migration assay and tranwell invasion assay, respectively. Representative images (100× magnification) are shown in D and the quantitative data are shown in E. The data were analyzed with GraphPad Prism 5.0, and are presented as means ± SEM, n = 3. *** p < 0.001 compared with the control group