Fig. 1.

In silico variant simulation workflow on real data using BamSurgeon: NA12878, NA12891 and NA24631 real datasets were spiked-in with non-overlapping variant subsets at different allele frequencies (ranging from 0.01 to 1) extracted from COSMIC database for S1, S2 and S3 simulation experiments. An additional simulation was performed using NA24631 dataset and non-COSMIC random mutations having VAF < =0.2. Text boxes coloured with blue borders represent steps embedded in NeoMutate workflow