Fig. 3.

Downregulation of ST6GAL1 attenuates proliferation and chemoresistance in T-ALL cell lines (a) The expression of ST6GAL1 was detected by qRT-PCR and western blot. b FCM was used to show the sialylation levels on the cell surface of transfected cell lines by FITC-SNA. c-e The proliferative ability was performed using CCK-8 assay, colony forming unit assay, immnuofluoresence analysis with Ki67. f The chemoresistance to ADR, VCR and Paclitaxel was detected by CCK-8 assays. g The IC₅₀ values were calculated. h The key apoptosis related molecules were determined by western blot. i FCM showed the apoptosis of transfected T-ALL cell lines in response to ADR. j The tumor tissues of nude mice were presented and calculated. k Tumor tissues were sectioned and stained with ST6GAL1 and Ki67 by IHC staining. Data were means ± SD of triplicate determinants (*P < 0.05)