Table 1.
Strains used, and presence of glycerol/diol dehydratase (GDH) and β–glucuronidase (B-GUS) encoding genes. The presence of gdh was predicted by metagenome analysis of human feces [24] and was confirmed for the used strains based on genome analysis (https://www.ncbi.nlm.nih.gov/genome/microbes/). The presence of b-gus was predicted by Dabek et al. [26] and McIntosh et al. [27]
| Strain name | Strain ID | gdh | b-gus |
|---|---|---|---|
| Blautia obeum | DSM 25238 | + | – |
| Eubacterium eligens | DSM 3376 | – | + |
| Eubacterium hallii | DSM 3353 | + | – |
| Faecalibacterium prausnitzii | DSM 17677 | – | + |
| Flavonifractor plautii | DSM 6740 | + | – |
| Intestinimonas butyriciproducens | DSM 26588 | + | – |
| Lactobacillus reuteri | DSM 20016 | + | – |
| Roseburia hominis | DSM 16839 | – | + |
| Roseburia intestinalis | DSM 14610 | – | + |
| Ruminococcus gnavus | ATCC 29149 | + | – |
| Veillonella dispar | ATCC 17748 | + | – |
| Bacteroides fragilis | ATCC 25285 | – | + |
| Citrobacter freundii | CB 36 | + | – |
| Klebsiella pneumoniae | CB 35 | + | – |
+/− gene encoding the indicated enzyme is present/absent in the representative genome. All strains are commercially available except C. freundii and K. pneumoniae, which were obtained in-house collection of Laboratory of Food Biotechnology, ETH Zurich