. 2019 May 16;19:99. doi: 10.1186/s12866-019-1483-x

Table 2.

Substrate utilization and metabolite production of single strains in the presence of glycerol or 1,2-propanediol. Strains were grown in anaerobic YCFA supplied with glycerol or 1,2-propanediol (1,2-PD) (both 50 mM) at 37 °C for 24 h. E. eligens did not grow and utilize glycerol and 1,2-PD. n.d. = not determined; n = number of biological replicates. Values are presented as mean ± standard deviation

Strain^a	Substrate	n	Substrate utilization (mM)	Metabolite production (mM)
Strain^a	Substrate	n	Substrate utilization (mM)	Formate	Acetate	Propionate	Butyrate	1,3-PD
B. obeum	1,2-PD	4	−17.9 ± 4.3^A*	0^A	1.5 ± 1.7^AB	5.2 ± 0.4^A	− 0.2 ± 0.6^A	n.d.
B. obeum	glycerol	6	−2.1 ± 1.2^a	0^a	1.5 ± 1.4^a	− 0.6 ± 0.4^a	0^a	0
E. hallii	1,2-PD	6	−46.6 ± 6.4^B	1.0 ± 0.8^A	− 1.6 ± 7.8^A	19.3 ± 8.0^B	4.3 ± 0.7^B	n.d.
E. hallii	glycerol	6	− 13.3 ± 3.0^b	0.7 ± 1.0^b	− 4.6 ± 2.5^b	0 ± 0.8^a	0 ± 0.8^a	1.9 ± 0.7^a
F. prausnitzii	1,2-PD	2	− 0.7^C	0^A	−1.4 ^A	0^C	1.5^A	n.d.
F. prausnitzii	glycerol	3	−1.6 ± 0.4^c	0.9 ± 1.6^a	1.3 ± 0.5^a	0.3 ± 0.2^a	0.3 ± 0.6^a	0 ^a
F. plautii	1,2-PD	4	−48.1 ± 1.2^B	0^A	2.2 ± 1.4^A	11.6 ± 3.9^B	4.1 ± 0.3^B	n.d.
F. plautii	glycerol	4	−9.1 ± 3.0^c	0^a	5.2 ± 0.7^c	−0.5 ± 0.4^a	4.3 ± 0.5^b	1.2 ± 0.7^a
I. butyriciproduens	1,2-PD	5	−1.2 ± 2.9^C	0^A	2.6 ± 2.6^A	− 0.1 ± 0.5^C	5.4 ± 0.6^B	n.d.
I. butyriciproduens	glycerol	3	− 0.8 ± 1.4^ac	0^a	2.5 ± 1.7^ac	−0.1 ± 0.1^a	5.3 ± 0.5^b	3.0 ± 0.1^b
L. reuteri	1,2-PD	3	−9.8 ± 4.2^C	0^A	−1.0 ± 1.1^A	0.9 ± 0.5^C	0^A	n.d.
L. reuteri	glycerol	3	−14.4 ± 8.8^b	0^a	−0.4 ± 1.1^a	0^a	0^a	5.2 ± 0^b
R. hominis	1,2-PD	3	0^D	0^A	0^A	0^C	0^A	n.g.
R. hominis	glycerol	5	−5.9 ± 1.1^c	0^a	−8.5 ± 1.3^d	−0.5 ± 0.2^a	7.4 ± 1.6^b	0
R. intestinalis	1,2-PD	3	−0.4 ± 0.4^C	0^A	− 0.4 ± 0.4^A	−0.3 ± 0.1^C	0.4 ± 0.2^A	n.d.
R. intestinalis	glycerol	3	−1.4 ± 0.6^ac	0^a	1.3 ± 1.0^a	0.4 ± 0.4^a	0.4 ± 0.2^a	0^a
R. gnavus	1,2-PD	3	−18.0 ± 5.1^A	1.1 ± 1.9^A	2.2 ± 1.4^AB	7.9 ± 2.4^A	0.2 ± 0.3^A	n.d.
R. gnavus	glycerol	3	− 1.4 ± 1.2^a	0^a	0.8 ± 1.4^ae	0^a	0^a	0.2 ± 0.3^a
V. dispar	1,2-PD	4	−1.7 ± 2.4^D	1.4 ± 1.6^A	0 ± 2.3^AB	0.6 ± 0.8^C	0^A	n.d.
V. dispar	glycerol	4	−4.4 ± 1.4^ac	6.6 ± 1.2^c	−2.4 ± 1.9^be	− 0.5 ± 0.5^a	−0.2 ± 0.4^a	0^a
B. fragilis	1,2-PD	4	−1.0 ± 1.4^C	0^A	−0.1 ± 1.6^A	0.3 ± 0.5^C	−0.4 ± 0.4^A	n.d.
B. fragilis	glycerol	3	− 1.0 ± 0.9^ac	0^a	0 ± 0.5^a	0.6 ± 0.6^a	0^a	0^a
C. freundii	1,2-PD	3	−1.4 ± 1.8^D	5.4 ± 0.6^B	7.2 ± 1.2^B	1.5 ± 0.6^D	0^A	n.d.
C. freundii	glycerol	3	−2.1 ± 3.2^a	6.0 ± 0.5^c	5.3 ± 2.0^c	−0.8 ± 0.8^a	0^a	0^a
K. pneumoniae	1,2-PD	3	− 42.7 ± 1.1^B	5.3 ± 0.1^B	3.1 ± 1.5^AB	16.7 ± 1.0^B	0^A	n.d.
K. pneumoniae	glycerol	3	− 48.7 ± 0.8^d	17.5 ± 1.2^d	10.7 ± 0.2^f	0^a	0^a	16.4 ± 3.3^c

*Different letters represent the significant differences (p < 0.05) on the substrate utilization or metabolite production between different strains using multiple comparisons with 2-way ANOVA. Capital letters are for 1,2-PD and the corresponding metabolites, small letters are for glycerol and the corresponding metabolites