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. 2019 May 16;38:200. doi: 10.1186/s13046-019-1179-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — FUT4 mediates the progression of CD34 + CD38-AML cell lines in vitro and in vivo. a The FUT4 mRNA (left panel) and protein (right panel) levels were detected by qRT-PCR and western blot in transfected LSCs-KG-1a/MOLM13 cells. b Altered LTL level of transfected LSCs cells was shown by flow cytometry. c Lower cell viability was measured by CCK8 assays in LSCs cells transfected with shFUT4. d Sphere formation ability was attenuated by shFUT4 transfection. e Immunoflurescence of Ki67 was decreased after knocking down FUT4. f The apoptotic rates of transfected LSCs-KG-1a cells were measured by flow cytometry. g The occurrence of apoptosis was determined by TUNEL assay. h Cleaved caspase3 and PARP levels were upregulated in shFUT4-LSCs by western blot. i Drug resistance to ADR, Ara-C and Pacilitaxel was determined by CCK8 assays. j Intensity of P-gp was weakening with down-regulating FUT4 in KG-1a cells by flow cytometry. k The tumorigenesis of transfected LSCs-KG-1a cells in vivo was presented with or without ADR treatment. (l) FUT4 and Ki67 levels were determined by immunohistochemistry staining. Data are the means ± SD of triplicate determinants (*P < 0.05)