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. 2019 Apr 8;8(4):94. doi: 10.3390/plants8040094

Figure 1.

Figure 1

Cytokinin induces GH3.5 and GH3.6 expressions. (A,C,E) Confocal images of five days after germination (dag) roots expressing pGH3.17–3xYFP, pGH3.5–3xYFP and pGH3.6–3xYFP constructs untreated (MS) and treated for four hours with 5 μM of cytokinin (+CK) (see Materials and Methods). Scale bar, 100 µm. (B,D,F) Mean Grey Value quantification of pGH3.17–3xYFP, pGH3.5–3xYFP and pGH3.6–3xYFP lines untreated (grey) and treated with cytokinin 5μM for four hours (+CK) (purple-pGH3.17–3xYFP; green-pGH3.5–3xYFP; orange-pGH3.6–3xYFP lines, respectively) at 5 dag where center lines show the medians. Box limits indicate the 25th and 75th percentiles as determined by R software. Whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, data points are plotted as open circles. Statistical significance: (B) Two biological replicates. p-value < 0.005, Student’s t-test, n = 16, 17 sample points, (D) two biological replicates. p-value < 0.05, Student’s t-test, n = 10, 9 sample points, (F) p-value < 0.005, Student’s t-test, n = 10, 10 sample points.