Table 16.

Staining of biopsies from dogs with chronic hepatitis

Category	Stain	Feature stained
Required	Hematoxylin and eosin	Nuclear and cytoplasmic features
	Rhodanine/Rubeanic acid	Copper
	Picrosirius red/Masson's trichrome	Collagen
Recommended	Gordon and Sweet	Reticulin
	Perl's	Iron
	Schmorl's	Lipofuscin
Situational	Periodic Acid Schiff (PAS)/diastase	Glycogen/non‐glycogen carbohydrates
	Oil Red O	Lipid
	Hall's stain	Bile
	Stains for infectious diseases, e.g; Ziehl Neelsen (acid fast), Fite's, Gomori methenamine silver (GMS), PAS, FISH	Organisms

Abbreviation: FISH, fluorescent in situ hybridization.