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. 2019 May 10;10:1113. doi: 10.3389/fmicb.2019.01113

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Copyright © 2019 Wang, Zheng, Sun, Feng, Gao, Fan, Chen, Jin and An.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DENV2-specific cytokine-secreting splenocytes in different immunization groups. Cytokine-secreting splenocytes were determined after stimulation with purified DENV2 particles. The positive splenocytes were counted and expressed as the mean SFU/3 × 10⁵ cells. (A) IL-4-positve and (B) IFN-γ-positive splenocytes with representative ELISPOT images, respectively. Data were expressed as mean + SD (n = 8). ^∗∗P < 0.01, ^∗∗∗P < 0.001.