Figure 1.

Lung inflammatory responses after chitin aspiration. (A–C), BALB/c mice were given 5 × 10³ purified chitin particles by aspiration on consecutive days, with BALF, lung homogenates, and serum harvested for analysis 24 h after the second challenge. (A) Representative flow cytometric dot plots of CD45^hi cells from control (saline) and chitin-aspirated mice. (B) Total number of BALF cells, Ly6G^hi neutrophils, Ly6G^loSiglecF^hiCD11c^lo eosinophils, and Ly6G^loSiglecF^hiCD11c^hi alveolar macrophages isolated from saline or chitin-aspirated mice as determined by flow cytometry. (C) Quantitative RT-PCR analysis of RNA from total lung homogenates of chitin-aspirated mice. N = 6–10/group. Data are shown as a summary of 2 experiments. Error bars in all figures represent standard error of mean. **p < 0.01. ***p < 0.001. ****p < 0.0001.