Skip to main content
. 2019 Aug;14(8):1394–1403. doi: 10.4103/1673-5374.253524

Figure 4.

Figure 4

Immunohistochemistry (brown color) for NeuN in the hippocampal formation of gerbils at different time after 5- and 15-min BCCAO.

(A) Sham operation; (B) 1 day after BCCAO, (C) 2 days after BCCAO, (D) 5 days after BCCAO. At 1 day after BCCAO, NeuN-ir neurons are not altered in all subregions. At 2 days after BCCAO, NeuN-ir neurons are decreased in the pyramidal layer (PL, asterisk) of the CA1 region alone. At 5 days after BCCAO, NeuN-ir neurons are rarely shown in the CA1 PL of gerbils from both 5- and 15-min BCCAO groups. In the 15-min BCCAO group, NeuN-ir are significantly decreased in number in the CA2/3 PL, dentate polymorphic layer (PoL) and lower granule cell layer (GCL, asterisk). Scale bar: 100 µm. (E–G) The mean number of NeuN-ir neurons in the CA1 (E), CA2/3 (F) and dentate gyrus (G) (n = 7 at each time after BCCAO; *P < 0.05, vs. the sham operated group, #P < 0.05, vs. the prior time point of corresponding BCCAO group, †P < 0.05, vs. the same time point of 5 min-BCCAO group). The bars indicate the mean ± SEM. BCCAO: Bilateral common carotid artery occlusion; OL: oriens layer; PL: polymorphic layer; RL: radiant layer; DG: dentate gyrus; GCL: granule cell layer; ML: molecular layer; min: minute.